Metabolic engineering of strains: from industrial-scale to lab-scale chemical production

by Sun J, Alper HS

Journal of Industrial Microbiology and Biotechnology, 2015 Mar;42(3):423-36. doi: 10.1007/s10295-014-1539-8. Epub 2014 Nov 21.

A plethora of successful metabolic engineering case studies have been published over the past several decades. Here, we highlight a collection of microbially produced chemicals using a historical framework, starting with titers ranging from industrial scale (more than 50 g/L), to medium-scale (5-50 g/L), and lab-scale (0-5 g/L). Although engineered Escherichia coli and Saccharomyces cerevisiae emerge as prominent hosts in the literature as a result of well-developed genetic engineering tools, several novel native-producing strains are gaining attention. This review catalogs the current progress of metabolic engineering towards production of compounds such as acids, alcohols, amino acids, natural organic compounds, and others.

Read the article